Synthesis and Deposition of Spore Coat Proteins during Sporulation ofBacillus megaterium
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چکیده
منابع مشابه
Changes in Microsporum gypseum mycelial wall and spore coat glycoproteins during sporulation and spore germination.
Ethylenediamine-soluble glycoproteins were extracted from isolated Microsporum gypseum hyphal walls during sporulation and from spore coats before and after germination. This study was carried out to identify a sporulation-specific cell wall protein that possibly served as a substrate for the alkaline protease which initiated the macroconidial germination of this fungus. Analyses revealed that ...
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In view of previously published reports of localized membrane growth in exponentially growing Bacillus megaterium and in sporulating Bacillus cereus, an attempt was made to describe phospholipid metabolism and the topology of membrane synthesis during sporulation in B. megaterium. The cells were pulsed with radioactive glycerol or acetate at the time of septum formation, and the specific activi...
متن کاملCharacterization, purification and synthesis of spore-coat protein in Bacillus megaterium KM.
The spore-coat fraction from Bacillus megaterium KM, when prepared by extraction of lysozyme-digested integuments with SDS (sodium dodecyl sulphate) and urea, contains three N-terminal residues and a major component of apparent mol.wt. 17500. Electron microscopy of this fraction shows it to consist of an ordered multilamellar structure similar to that which forms the coat region of intact spore...
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Spore coat protein synthesis in submerged pseudoplasmodia of Dictyostelium discoideum is dependent on the presence of a low relative molecular mass extracellular factor, SPIF, the activity of which can be mimicked by methionine. In vitro translation and northern blot analysis revealed that the level of spore coat protein mRNA in pseudoplasmodia incubated in the absence of methionine is little d...
متن کاملDifferential synthesis of spore coat proteins in prespore and prestalk cells of Dictyostelium.
The major spore coat proteins (SP60, SP70, and SP96) of Dictyostelium discoideum have been analyzed by using two-dimensional nonequilibrium pH gradient electrophoresis. These proteins have been characterized with respect to electrophoretic behavior, metachromatic staining with silver, and posttranslational modifications; these techniques allow unambiguous identification of these proteins in sil...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1985
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1985.tb00905.x